Pillai, Beena and Verma, Jiyoti and Abraham, Anju and Francis, Princy and Kumar, Yadunanda and Tatu, Utpal and Brahmachari, Samir K and Sadhale, Parag P (2003) Whole genome expression profiles of yeast RNA polymerase II core subunit, Rpb4, in stress and nonstress conditions. In: Journal of Biological Chemistry, 278 (5). pp. 3339-3346.
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Organisms respond to environmental stress by adopting changes in gene expression at the transcriptional level. Rpb4, a nonessential subunit of the core RNA polymerase II has been proposed to play a role in non-stress-specific transcription and in the regulation of stress response in yeast. We find that in addition to the temperature sensitivity of the null mutant of Rpb4, diploid null mutants are also compromised in sporulation and show morphological changes associated with nitrogen starvation. Using whole genome expression analysis, we report here the effects of Rpb4 on expression of genes during normal growth and following heat shock and nutritional starvation. Our analysis shows that Rpb4 affects expression of a small yet significant fraction of the genome in both stress and normal conditions. We found that genes involved in galactose metabolism were dependent on the presence of Rpb4 irrespective of the environmental condition. Rpb4 was also found to affect the expression of several other genes specifically in conditions of nutritional starvation. The general defect in the absence of Rpb4 is in the expression of metabolic genes, especially those involved in carbon metabolism and energy generation. We report that various stresses are affected by RPB4 and that on overexpression the stress-specific activators can partially rescue the corresponding defects.
|Item Type:||Journal Article|
|Additional Information:||The copyright of this article belongs to The American Society for Biochemistry and Molecular Biology, Inc.|
|Keywords:||Genes; RNA; Stress analysis; Nitrogen; Metabolism; Morphology|
|Department/Centre:||Division of Biological Sciences > Biochemistry
Division of Biological Sciences > Microbiology & Cell Biology
|Date Deposited:||16 May 2007|
|Last Modified:||22 Feb 2012 09:06|
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