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A Mycobacterium smegmatis gyrase B specific monoclonal antibody reveals association of gyrase A and B subunits in the cell

Manjunatha, UH and Somesh, BP and Nagaraja, V and Visweswariah, Sandhya S (2001) A Mycobacterium smegmatis gyrase B specific monoclonal antibody reveals association of gyrase A and B subunits in the cell. In: FEMS Microbiogy Letters, 194 . pp. 87-92.

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Abstract

DNA gyrase is a unique topoisomerase, which plays important roles in macromolecular events like DNA replication, transcription and genetic recombination. In this study a high affinity monoclonal antibody to the gyrase B (GyrB) subunit of Mycobacterium smegmatis was characterized, which did not cross-react with either the Escherichia coli GyrB subunit or with GyrB subunits from other mycobacterial species. The antibody recognized an epitope in the N-terminus, novobiocin-binding domain of GyrB. Immunoprecipitation of gyrase from M. smegmatis cell lysate revealed an association, mediated by ionic interactions, of gyrase A and GyrB subunits in the cell. This antibody is a valuable tool for structure^function analysis and immunocytological studies of mycobacterial DNA gyrase. fl 2001 Federation of European Microbiological Societies.

Item Type: Journal Article
Keywords: Mycobacterium;DNA gyrase;Monoclonal antibody;Epitope mapping;Novobiocin;Surface plasmon resonance
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 01 Sep 2004
Last Modified: 19 Sep 2010 04:14
URI: http://eprints.iisc.ernet.in/id/eprint/1098

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