Saravanan, Matheshwaran and Elango, Kathirvel and Chandrashekaran, Siddamadappa and Anand, Narayanaswamy and Nagaraja, Valakunja (2003) Catechol dioxygenase is an insensitive reporter for transcription in Mycobacterium smegmatis. In: Current Science, 85 (2). pp. 188-191.
Although restriction enzymes are widely distributed in nature, many bacterial genera are yet to be explored for the presence of this important class of enzymes. We have purified and characterized a new type II restriction endonuclease, OfoI from a nonheterocyst cyanobacterium Oscillatoria foreaui. The recognition sequence has been determined by primer extension analysis. The purified enzyme OfoI recognizes and cleaves the palindromic hexanucleotide 5¢-C¯YCGRG-3¢, generating 5¢-protruding ends.
|Item Type:||Journal Article|
|Keywords:||nonheterocystous cyanobacterium Oscillatoria foreaui|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||22 Jul 2004|
|Last Modified:||19 Sep 2010 04:14|
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