Mammalian Brain Plasma Membranes: Isolation, Enzymatic, and Characterization

Krishnan, KS and Balaram, P (1976) Mammalian Brain Plasma Membranes: Isolation, Enzymatic, and Characterization. In: Experimental Cell Research, 101 . pp. 299-306.

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Abstract

Two variants of a simplified procedure for the isolation of plasma membrane fractions from monkey and rat brains, are described. The preparations showed marked enrichments in the marker enzymes, (Na+ - K+) adenosine triphosphatase, acetylcholinesterase, 5’-nucleotidase and adenylate cyclase. Lipid analysis and a protein electrophoretic pattern are presented. An enzymatic check has been made to assess for contamination by other cellular organelles. The amino acid composition of brain membrane proteins show a resemblance to the reported composition of erythrocyte ghost proteins but differ from myelin proteins.

Item Type:	Journal Article
Additional Information:	Copyright for this article belongs to Academic Press
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	26 Jul 2004
Last Modified:	19 Sep 2010 04:14
URI:	http://eprints.iisc.ernet.in/id/eprint/1159

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