Gupta, Sarika and Singh, Anchal and Yadav, Marshleen and Singh, Kalyan and Rathaur, Sushma (2007) MALDI mass sequencing and characterization of ﬁlarial glutathione-S-transferase. In: Biochemical and Biophysical Research Communications, 356 (2). pp. 381-385.
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Glutathione-S-transferase has been detected in the somatic extract and excretory–secretory products of different life stages of Setaria cervi, a bovine ﬁlarial parasite. The enzyme was subjected to MALDI-TOF followed by mass spectrometry and the nearest match found was Pleuronectes platessa GST. Molecular mass of the puriﬁed enzyme was $\approx 26$ kDa as determined by SDS–PAGE and MALDI-TOF. Setaria cervi GST exhibited high activity towards 1-chloro-2,4-dinitrobenzene and ethacrynic acid. Kinetic analysis with respect to 1-chloro-2,4-dinitrobenzene and glutathione as substrate revealed a $K_m$ of 2.22 mM and 0.61 mM, respectively. The activity was inhibited signiﬁcantly by Cibacron blue and $\alpha$ -tocopherol.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier.|
|Keywords:||MALDI mass;Glutathione-S-transferase;Filariasis;Setaria cervi|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||30 Jul 2007|
|Last Modified:||15 Jan 2013 06:43|
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