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MALDI mass sequencing and characterization of filarial glutathione-S-transferase

Gupta, Sarika and Singh, Anchal and Yadav, Marshleen and Singh, Kalyan and Rathaur, Sushma (2007) MALDI mass sequencing and characterization of filarial glutathione-S-transferase. In: Biochemical and Biophysical Research Communications, 356 (2). pp. 381-385.

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Official URL: http://dx.doi.org/10.1016/j.bbrc.2007.02.147

Abstract

Glutathione-S-transferase has been detected in the somatic extract and excretory–secretory products of different life stages of Setaria cervi, a bovine filarial parasite. The enzyme was subjected to MALDI-TOF followed by mass spectrometry and the nearest match found was Pleuronectes platessa GST. Molecular mass of the purified enzyme was $\approx 26$ kDa as determined by SDS–PAGE and MALDI-TOF. Setaria cervi GST exhibited high activity towards 1-chloro-2,4-dinitrobenzene and ethacrynic acid. Kinetic analysis with respect to 1-chloro-2,4-dinitrobenzene and glutathione as substrate revealed a $K_m$ of 2.22 mM and 0.61 mM, respectively. The activity was inhibited significantly by Cibacron blue and $\alpha$ -tocopherol.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier.
Keywords: MALDI mass;Glutathione-S-transferase;Filariasis;Setaria cervi
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 30 Jul 2007
Last Modified: 15 Jan 2013 06:43
URI: http://eprints.iisc.ernet.in/id/eprint/11620

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