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Site-directed mutagenesis using a single mutagenic oligonucleotide and DpnI digestion of template DNA

Shenoy, Avinash R and Visweswariah, Sandhya S (2003) Site-directed mutagenesis using a single mutagenic oligonucleotide and DpnI digestion of template DNA. In: Analytical Biochemistry, 319 (2). pp. 335-336.

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Abstract

Schematic of the mutagenesis protocol. PCR is carried out as described and a single-stranded nicked DNA molecule containing the desired mutation is left at the end of the reaction following digestion with DpnI. This is transformed into competent DH10B cells to obtain plasmid DNA with the desired mutation.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science
Keywords: mutagenesis;Escherichia coli cells;single mutagenic oligonucleotide
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)
Date Deposited: 01 Oct 2007
Last Modified: 19 Sep 2010 04:39
URI: http://eprints.iisc.ernet.in/id/eprint/11976

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