Thomas, Celestine J and Surolia, Avadhesha (2000) Kinetic Analysis of the Binding of Ulex europeas Agglutinin 1 (UEA 1) to H-Antigenic Fucolipid. In: Archives of Biochemistry and Biophysics, 374 (1). pp. 8-12.
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The kinetics and thermodynamics of Ulex europeas agglutinin 1-H-antigenic determinant was studied by monitoring the binding of the lectin to H-antigenic fucolipid incorporated in liposomes tethered to the sensor surface by surface plasmon resonance. The second-order rate constants for the interactions are several order of magnitude slower than the diffusion controlled reactions. Analyses of activation parameters reveals that these slow second-order rate constants are a consequence of an unfavorable activation enthalpy term.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Academic Press|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||15 Oct 2007|
|Last Modified:||19 Sep 2010 04:39|
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