Rohini, VK and Rao, Sankara K (2000) Transformation of peanut (Arachis hypogaea L.): a non-tissue culture based approach for generating transgenic plants. In: Plant Science, 150 (1). pp. 41-49.
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Transgenic peanut (Arachis hypogaea L. cv. TMV-2) plants have been produced by a tissue culture-independent Agrobacterium tumefaciens-mediated transformation procedure. Embryo axes of mature seeds with one cotyledon excised were incubated on Murashige and Skoog (MS) gelled medium for 2 days, then pricked randomly with a sterile needle and infected by immersion in a suspension of Agrobacterium in Winans’ AB medium that was added with wounded tobacco leaf extract. Agrobacterium strain LBA 4404 harboring the binary vector pKIWI105 that carries the genes for $\beta$-glucuronidase (GUS) and neomycin phosphotransferase (NPT II) was used for transformation. Following a 16 h infection, 18 h decontamination with cefotaxime and germination and growth on soilrite for 16 days under growth room conditions, the seedlings were transferred to greenhouse. About 3.3% of the seedlings were GUS positive as determined by histochemical assay and by PCR analysis. Molecular characterization of primary transformants as well as the $T_1$ and $T_2$ generation plants has shown that the method ensured insertion, expression and inheritance of foreign genes in peanut.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier.|
|Keywords:||Arachis hypogaea L.;Genetic transformation;Tissue culture-independent method;Agrobacterium tumefaciens;β-Glucuronidase;Neomycin phosphotransferase|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||01 Oct 2007|
|Last Modified:||19 Sep 2010 04:39|
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