Kumar, Ranjith CT and Manoharan, M and Prasad, Krishna S and Cherian, Shoba and Umashankar, M and Sita, Lakshmi G and Savithri, HS (1999) Engineering resistance against physalis mottle tymovirus by expression of the coat protein and 3' noncoding region. In: Current Science, 77 (11). pp. 1542-1547.
A 748 nucleotides cDNA fragment corresponding to the 3' terminal of physalis mottle virus, PhMV (formerly known as belladonna mottle virus) ($\#$Y16104) genomic RNA encompassing the tymobox, coat protein ORF and 3' noncoding region was cloned into the binary vector pKYLX 71 35 $S^2$ and introduced into N. tabacum cv. Havana plants using Agrobacterium-mediated transformation. The R0 transgenic plants showed accumulation of coat protein which self-assembled into capsids in vivo. The transgenic R1 and R2 plants showed delay in symptom expression and virus accumulation upon challenge with PhMV. 55 and 65% of the plants showed no detectable symptoms in the R1 and R2 transgenic plants respectively, when challenged with 10 $\mu$g/ml virus. Further, no detectable symptoms were observed in 75% and 25% of the R1 and R2 transgenic plants respectively, after 50 days of post infection when challenged with 10 $\mu$g/ml RNA. Thus the expression of PhMV coat protein and 3' noncoding sequence confers a high level of resistance against PhMV infection.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Indian Academy of Sciences|
|Keywords:||Molecular Genetics;Biochemistry and Molecular Biophysics;Infection|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||16 Oct 2007|
|Last Modified:||19 Sep 2010 04:40|
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