Kondaiah, Paturu and Taira, Masanori and Vempati, Uma Devi and Dawid, Igor B (2000) Transforming growth factor-$\beta 5$ expression during early development of Xenopus laevis. In: Mechanisms of Development, 95 (1-2). pp. 207-209.
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Members of the transforming growth factor-\beta $(TGF-\beta)$ superfamily play various roles during development in both vertebrates and invertebrates. Two isoforms, $TGF-\beta 2$ and -$\beta 5$, have been isolated from Xenopus laevis. We describe here the localization of $TGF-\beta 5$ mRNA in early embryos of X. laevis, assessed by whole-mount in situ hybridization. The first detectable expression of $TGF-\beta 5$ was seen in the stage 14 embryo at the posterior tip of notochord, which continued to later stages, accompanied by the expression in bilateral regions of posterior wall in the tail region next to the notochord. At later stages, transient expression was seen in the cement gland (around stage 21) and in the somites (stages 24–27). In addition, expression was present in the branchial arches (stage 29–36) and olfactory placodes (stage 36).
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier.|
|Keywords:||TGF-β;Xenopus laevis;In situ whole-mount hybridization;mRNA;Gene expression;Somites;Cement gland;Branchial arches;Notochord;Olfactory placode|
|Department/Centre:||Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)|
|Date Deposited:||16 Oct 2007|
|Last Modified:||19 Sep 2010 04:40|
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