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Sodium channel modulating activity in a delta-conotoxin from an Indian marine snail

Sudarslal, S and Majumdar, Sriparna and Ramasamy, P and Dhawan, Ritu and Pal, Prajna P and Ramaswami, Mani and Lala, Anil K and Sikdar, SK and Sarma, Siddhartha P and Krishnan, KS and Balaram, P (2003) Sodium channel modulating activity in a delta-conotoxin from an Indian marine snail. In: FEBS Letters, 553 (1-2). pp. 209-212.

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Abstract

A 26 residue peptide (Am 2766) with the sequence CKQAGESCDIFSQNCCVG-TCAFICIE-NH2 has been isolated and purified from the venom of the molluscivorous snail, Conus amadis, collected off the southeastern coast of India. Chemical modification and mass spectrometric studies establish that Am 2766 has three disulfide bridges. C-terminal amidation has been demonstrated by mass measurements on the C-terminal fragments obtained by proteolysis. Sequence alignments establish that Am 2766 belongs to the delta-conotoxin family. Am 2766 inhibits the decay of the sodium current in brain rNav1.2a voltage-gated Na+ channel, stably expressed in Chinese hamster ovary cells. Unlike delta-conotoxins have previously been isolated from molluscivorous snails, Am 2766 inhibits inactivation of mammalian sodium channels.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to Elsevier Science Ltd
Keywords: delta-Conotoxin;Sodium channel;Conus peptide;Mass spectrometry;Conus amadis
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 29 Jul 2004
Last Modified: 19 Sep 2010 04:14
URI: http://eprints.iisc.ernet.in/id/eprint/1212

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