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Differential Effects of Tri-n-Butylstannyl Benzoates on Induction of Apoptosis in K562 and MCF-7 Cells

Ray, D and Sarma, KD and Antony, A (2000) Differential Effects of Tri-n-Butylstannyl Benzoates on Induction of Apoptosis in K562 and MCF-7 Cells. In: IUBMB Life, 49 (6). pp. 519-525.

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Abstract

Tributyltin compounds have been shown to induce apoptosis by causing extracellular $Ca^{2+}$ influx and generating reactive oxygen species (ROS). Several organotin compounds were reported to have differential cytotoxicity on various human cell lines depending on the length of the alkyl chain. In this report, the cytotoxic effects of three tri-n-butylstannyl (halo)benzoate compounds-tri-n-butylstannyl benzoate (TBSB), tri-n-butylstannyl 2,6-difluorobenzoate (TBSDFB) and tri-n-butylstannyl-2-iodobenzoate (TBSIB)-were studied on lymphocytic cells of human leukemic K562 lineage and epithelial cells of human breast cancer MCF-7 cells lineage. K562 cells were found to be more sensitive to these compounds than MCF-7 cells. Although the induction of apoptosis by the above compounds in K562 cells resulted from the extracellular $Ca^{2+}$ influx and the generation of ROS, the initial amount of extracellular $Ca^{2+}$ influx was greater in TBSB-treated K562 cells than the cells treated with either TBSDFB or TBSIB. Similarly, DNA fragmentation by endonucleases was observed as an early event in TBSB-treated K562 cells, which might be correlated with the initially greater extracellular $Ca^{2+}$ influx. In contrast, MCF-7 cells were found to undergo apoptosis mainly because of the generation of ROS. The present results suggest that the differential effects of tributyltin compounds on induction of apoptosis in K562 and MCF-7 cells are largely attributable to the extent of extracellular $Ca^{2+}$ influx.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Taylor and Francis Ltd.
Keywords: APOPTOSIS;CA2+;INFLUX;K562;MCF-7;ROS;TRI-N-BUTYLSTANNYL;BENZOATES
Department/Centre: Division of Chemical Sciences > Organic Chemistry
Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 09 Oct 2007
Last Modified: 19 Sep 2010 04:40
URI: http://eprints.iisc.ernet.in/id/eprint/12157

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