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Structural Studies on the Empty Capsids of Physalis Mottle Virus

Sri Krishna, S and Sastri, M and Savithri, HS and Murthy, MRN (2001) Structural Studies on the Empty Capsids of Physalis Mottle Virus. In: Journal of Molecular Biology, 307 (4). pp. 1035-1047.

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Abstract

The three-dimensional crystal structure of the empty capsid of Physalis mottle tymovirus has been determined to 3.2 \AA resolution. The empty capsids crystallized in the space group P1, leading to 60-fold non-crystallographic redundancy. The known structure of Physalis mottle virus was used as a phasing model to initiate the structure determination by real-space electron-density averaging. The main differences between the structures of the native and the empty capsids were in residues 10 to 28 of the A-subunit, residues 1 to 9 of the B-subunit and residues 1 to 5 of the C-subunit, which are ordered only in the native virus particles. An analysis of the subunit disposition reveals that the virus has expanded radially outward by $\sim 1.8$ \AA in the empty particles. The A-subunits move in a direction that makes $10^o$ to the icosahedral 5-fold axes of symmetry. The B and C-subunits move along vectors making $12^o$ and $15^o$ to the quasi 6-fold axes. The quaternary organization of the pentameric and hexameric capsomeres are not altered significantly. However, the pentamer-hexamer contacts are reduced. Therefore, encapsidation of RNA appears to cause a reduction in the particle radius concomittant with the ordering of the N-terminal arm in the three subunits. These structural changes in Physalis mottle virus appear to be larger than the corresponding changes observed in viruses for which both the empty and full particle structures have been determined.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Academic Press.
Keywords: Assembly/disassembly;Capsid stability;Physalis mottle tymovirus;Viral structure;X-ray diffraction
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Biochemistry
Date Deposited: 09 Oct 2007
Last Modified: 19 Sep 2010 04:40
URI: http://eprints.iisc.ernet.in/id/eprint/12172

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