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Identification of Two Novel Zinc Finger Modules and Nuclear Localization Signal in Rat Spermatidal Protein TP2 by Site-directed Mutagenesis

Meetei, Amom R and Ullas, Kolathur S and Rao, Manchanahalli RS (2000) Identification of Two Novel Zinc Finger Modules and Nuclear Localization Signal in Rat Spermatidal Protein TP2 by Site-directed Mutagenesis. In: Journal of Biological Chemistry, 275 (49). pp. 38500-38507.

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Abstract

Spermatidal protein TP2, which appears transiently during stages 12-16 of mammalian spermiogenesis, is a DNA condensing zinc metalloprotein with a preference to GC-rich DNA. We have carried out a detailed site-directed mutagenesis analysis of rat spermatidal protein TP2 to delineate the amino acid residues involved in coordination with two atoms of zinc. Two zinc fingers modules have been identified involving 4 histidine and 4 cysteine residues, respectively. The modular structure of the two zinc fingers identified in TP2 define a new class of zinc finger proteins that do not fall into any of the known classes of zinc fingers. Transfection experiments with COS-7 cells using wild type and the two zinc finger pocket mutants have shown that TP2 preferentially localizes to nucleolus. The nuclear localization signal in TP2 was identified to be $^{87}GKVSKRKAV^{95}$ present in the C-terminal third of TP2 as a part of an extended NoLS sequence.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to American Society for Biochemistry and Molecular Biology
Keywords: Biochemistry;Molecular Biophysics;Methods and Techniques;Respiratory System;Respiration
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 24 Oct 2007
Last Modified: 19 Sep 2010 04:40
URI: http://eprints.iisc.ernet.in/id/eprint/12192

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