ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Specific interaction between the ribosome recycling factor and the elongation factor G from Mycobacterium tuberculosis mediates peptidyl-tRNA release and ribosome recycling in Escherichia coli

Rao, Arasada Rajeswara and Varshney, Umesh (2001) Specific interaction between the ribosome recycling factor and the elongation factor G from Mycobacterium tuberculosis mediates peptidyl-tRNA release and ribosome recycling in Escherichia coli. In: The EMBO Journal, 20 (11). pp. 2977-2986.

[img] PDF
Specific_interaction_between_the_ribosome.pdf
Restricted to Registered users only

Download (429Kb) | Request a copy

Abstract

Once the translating ribosomes reach a termination codon, the nascent polypeptide chain is released in a factor-dependent manner. However, the P-site-bound deacylated tRNA and the ribosomes themselves remain bound to the mRNA (post-termination complex). The ribosome recycling factor (RRF) plays a vital role in dissociating this complex. Here we show that the Mycobacterium tuberculosis RRF (MtuRRF) fails to rescue Escherichia coli LJ14, a strain temperature-sensitive for RRF $(frr^{ts})$. More interestingly, co-expression of M.tuberculosis elongation factor G (MtuEFG) with MtuRRF rescues the $frr^{ts} $ strain of E.coli. The simultaneous expression of MtuEFG is also needed to cause an enhanced release of peptidyl-tRNAs in E.coli by MtuRRF. These observations provide the first genetic evidence for a functional interaction between RRF and EFG. Both the in vivo and in vitro analyses suggest that RRF does not distinguish between the translating and terminating ribosomes for their dissociation from mRNA. In addition, complementation of E.coli PEM100 $(fusA^{ts})$ with MtuEFG suggests that the mechanism of RRF function is independent of the translocation activity of EFG.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to European Molecular Biology Organization.
Keywords: frr;fusA;Mycobacteria;Peptidyl-tRNA;Termination of protein synthesis
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 15 Oct 2007
Last Modified: 19 Sep 2010 04:40
URI: http://eprints.iisc.ernet.in/id/eprint/12276

Actions (login required)

View Item View Item