Ojha, Anil Kr and Varma, Saaket and Chatterji, Dipankar (2002) Synthesis of an unusual polar glycopeptidolipid in glucose-limited culture of Mycobacterium smegmatis. In: Microbiology, 148 (10). pp. 3039-3048.
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There has been a general understanding that Mycobacterium smegmatis produces only apolar glycopeptidolipid (GPL), similar in structure to serovar non-specific GPL of Mycobacterium avium. In this study, synthesis of polar GPL in carbon-starved M. smegmatis is reported. Mass spectrometric analysis suggests the polar GPL to be a hyperglycosylated species. The earlier structural studies of polar GPLs from M. avium have invariably shown the presence of an oligosaccharide appendage to D-allo-Thr. However, a further chemical analysis using \beta-elimination of the newly found polar GPL in M. smegmatis shows that the molecule still contains a monosaccharide at the D-allo-Thr, thus suggesting a new form of polar GPL.
|Item Type:||Journal Article|
|Additional Information:||Copyright belongs to this article is Society for General Microbiology.|
|Keywords:||Polar glycopeptidolipid;Carbon starvation|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||22 Oct 2007|
|Last Modified:||19 Sep 2010 04:40|
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