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Thermodynamics of hCG–monoclonal antibody interaction: an analysis of real time kinetics data obtained using radiolabeled hCG probe

Banerjee, Ashish and Selvi, Tamil P and Murthy, Gundlupet Satyanarayana (2002) Thermodynamics of hCG–monoclonal antibody interaction: an analysis of real time kinetics data obtained using radiolabeled hCG probe. In: Biochimica et Biophysica Acta (BBA) - General Subjects, 1572 (1). pp. 31-36.

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Abstract

A thermodynamic analysis of the interaction of $^{125}$ I-labeled human chorionic gonadotropin (IhCG) with two of its monoclonal antibodies (MAbs) was carried out. The dissociation profile of IhCG–MAb complex conforms to a two-step model. vant Hoff enthalpies were calculated with the $K_A$ (equilibrium constant) values obtained from dissociation at different temperatures. Free energy and entropy changes were calculated using the standard equations. $\Delta H$ values for one of the MAbs, viz. VM7 were favorable at temperatures beyond $30^oC$. Interestingly, the $\Delta S$ values were also favorable at all temperatures. In the case of MAb VM4a, however, the interaction throughout the temperature range was driven by large favorable entropic contributions, indicating the importance of hydrophobic interaction in the binding of this MAb to hCG. The energetics of the interaction of these two monoclonals with hCG is discussed.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier.
Keywords: Real time kinetics;Antigen–antibody;Human chorionic gonadotropin;Thermodynamics
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)
Date Deposited: 25 Oct 2007
Last Modified: 19 Sep 2010 04:40
URI: http://eprints.iisc.ernet.in/id/eprint/12349

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