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Enhanced functional and structural domain assignments using remote similarity detection procedures for proteins encoded in the genome of Mycobacterium tuberculosis H37Rv

Namboori, Seema and Mhatre, Natasha and Sujatha, Sentivel and Srinivasan, Narayanaswamy and Pandit, Shashi Bhushan (2004) Enhanced functional and structural domain assignments using remote similarity detection procedures for proteins encoded in the genome of Mycobacterium tuberculosis H37Rv. In: Journal of Biosciences, 29 (3). pp. 245-259.

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Abstract

The sequencing of the Mycobacterium tuberculosis (MTB) H37Rv genome has facilitated deeper insights into the biol. of MTB, yet the functions of many MTB proteins are unknown. We have used sensitive profile-based search procedures to assign functional and structural domains to infer functions of gene products encoded in MTB. These domain assignments have been made using a compendium of sequence and structural domain families. Functions are predicted for 78% of the encoded gene products. For 69% of these, functions can be inferred by domain assignments. The functions for the rest are deduced from their homology to proteins of known function. Superfamily relationships between families of unknown and known structures have increased structural information by \sim 11%. Remote similarity detection methods have enabled domain assignments for 1325 'hypothetical proteins'. The most populated families in MTB are involved in lipid metabolism entry and survival of the bacillus in host. Interestingly, for 353 proteins, which we refer to as MTB-specific, no homologs have been identified. Numerous, previously unannotated, hypothetical proteins have been assigned domains and some of these could perhaps be the possible chemotherapeutic targets. MTB-specific proteins might include factors responsible for virulence. Importantly, these assignments could be valuable for experimental endeavors. The detailed results are publicly available at http://hodgkin.mbu.iisc.ernet.in/ \sim dots

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Indian Academy of Sciences.
Keywords: Genome data analysis;hypothetical proteins, Mycobacterium tuberculosis;protein structures;structural and functional domains
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Centre for Ecological Sciences
Date Deposited: 31 Oct 2007
Last Modified: 19 Sep 2010 04:41
URI: http://eprints.iisc.ernet.in/id/eprint/12377

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