Kumar, Madan A and Nayak, R (1990) Glycosidic bond cleavage of 5-fluoro-2'-deoxyuridine and 5-fluorouridine by amino acyl-tRNA synthetases. In: Biochemical and Biophysical Research Communications, 173 (2). pp. 731-735.
Restricted to Registered users only
Download (493Kb) | Request a copy
Lysyl-tRNA synthetase isolated from rat liver cleaves glycosidic bond of 5-Fluorouridine and 5-Fluoro-2'-deoxyuridine to generate 5-Fluorouracil. The generation of 5-Fluorouracil was monitored by cellulose thin layer chromatography and by spectrophotometry. The enzyme was found to be 1.4 fold more efficient in cleaving the glycosidic bond of 5-Fluoro-2'-deoxyuridine than 5-Fluorouridine.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Academic Press, Inc.|
|Keywords:||Glycosidic bond cleavage;amino acyl-tRNA;chromatography;spectrophotometry|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||10 Dec 2007|
|Last Modified:||19 Sep 2010 04:41|
Actions (login required)