Gopalakrishnan, MV and Padayatty, JD (1990) A bacteriophage lytic enzyme gene as a marker for gene transfer and expression. In: Current Science, 59 (7). pp. 386-387.
The plasmid pBR322 bearing the lytic enzyme gene of colitis bacteriophage was injected into 10 sets of 10 onion bulbs each, at multiple points, and germinated for 7 days. The lytic enzyme was detected in the extracts of 0-60%of the bulbs in each set by turbidometric assay. This lyticenzyme gene can be used as a marker gene for gene transfer in plants and animals and in chimaeric gene constructs to study regulation of gene expression.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Indian Academy of Sciences|
|Keywords:||bacteriophage;lytic enzyme gene;turbidometric|
|Department/Centre:||Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)
Division of Biological Sciences > Biochemistry
|Date Deposited:||28 Dec 2007|
|Last Modified:||19 Sep 2010 04:41|
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