# A spectrophotometric method for detection of $5^\prime$-fluorouracil in plasma

Kumar, Madan A and Nayak, R (1990) A spectrophotometric method for detection of $5^\prime$-fluorouracil in plasma. In: Biochemical and Biophysical Research Communications, 169 (3). pp. 1153-1157.

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## Abstract

A quantitative method using spectrophotometry was devised for the detection of $5^\prime$-Fluorouracil(FUra) in plasma. Plasma samples obtained at different time intervals from drug treated rabbits were deproteinised using phenol. The aqueous phase was lyophilised and dissolved in 0.01 N NaOH and absorption was monitored at 305 nm. Levels of FUra in the plasma were calculated by comparing with a standard graph generated from known concentrations of FUra. As low as 1 $\mu$g of the drug per ml of plasma could be detected by this method.

Item Type: Journal Article Copyright of this article belongs to Academic Press, Inc. spectrophotometric;fluorouracil;plasma Division of Biological Sciences > Microbiology & Cell Biology 20 Feb 2008 19 Sep 2010 04:41 http://eprints.iisc.ernet.in/id/eprint/12670