Zintrons in rat \alpha-lactalbumin gene

Meera, G and Ramesh, N and Brahmachari, Samir K (1989) Zintrons in rat \alpha-lactalbumin gene. In: FEBS Letters, 251 (1-2). pp. 245-249.

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Abstract

The eukaryotic genome is characterised by the occurrence of a large amount of repetitive DNA which exists within and around coding sequences. Random repeats of $(TG)_n$ sequences have been observed in several introns. In this paper we show that $(TG)_{14}$and $(TG)_{24}$ sequences present in the third intron of rat \alpha-lactalbumin gene adopt left-handed Z-helices under varying superhelical densities. The overall sequence of the parent plasmid further influenced the level of supercoiling at which the B to Z transition could be induced in $(TG)_n$ sequences. Such Z-potential intervening sequences. (zintrons) could act as buffers to maintain desired levels of supercoiling near the transcribed sequences.

Item Type:	Journal Article
Additional Information:	Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:	Lactalbumin, α-;Intron sequence;DNA, Z-;DNA supercoiling;(Rat)
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	13 Feb 2008
Last Modified:	19 Sep 2010 04:42
URI:	http://eprints.iisc.ernet.in/id/eprint/12919

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