ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Identification and molecular characterization of a biotin-binding protein distinct from avidin of chicken egg white and comparison with yolk biotin-binding protein

Seshagiri, Polani B and Adiga, Radhakantha P (1987) Identification and molecular characterization of a biotin-binding protein distinct from avidin of chicken egg white and comparison with yolk biotin-binding protein. In: Biochimica et Biophysica Acta (BBA) - General Subjects, 926 (3). pp. 321-330.

[img] PDF
fulltext.pdf - Published Version
Restricted to Registered users only

Download (948Kb) | Request a copy
Official URL: http://dx.doi.org/10.1016/0304-4165(87)90218-2

Abstract

By immunol. and biochem. methods a biotin-binding protein, distinct from avidin, was shown to be present in chicken egg white. This vitamin-binding protein (mol. wt. 67,000) bound [14C]biotin, displayed thermally induced biotin exchange reaction, and exhibited gross immunol. cross-reactivity with the purified yolk biotin-binding protein. In vitro labeling of sol. proteins with radioactive amino acids in the oviduct tissue explants from estrogenized chicks revealed that .apprx.2% of the total radioactive proteins immunopptd. with anti-yolk biotin-binding protein antibodies. The protein was purified to homogeneity by ion-exchange chromatog. on DEAE-cellulose and biotin-AH Sepharose affinity chromatog. The purified protein specifically bound [14C]biotin, and exhibited complete immunol. homol. with the yolk biotin-binding protein but not with avidin. Its electrophoretic mobility (at pH 8.3), acidic nature, biotin-binding characteristics, immunol. cross-reactivity, and tryptic peptide maps were very similar to those of yolk biotin-binding protein, and not avidin.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Biotin binding protein;Avidin (Chicken egg)
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 04 Mar 2008
Last Modified: 01 Mar 2012 07:31
URI: http://eprints.iisc.ernet.in/id/eprint/13155

Actions (login required)

View Item View Item