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Purification of a circulatory riboflavin carrier protein from pregnant bonnet monkey (M. radiata): comparison with chicken egg vitamin carrier

Visweswariah, Sandhya S and Adiga, Radhakantha P (1987) Purification of a circulatory riboflavin carrier protein from pregnant bonnet monkey (M. radiata): comparison with chicken egg vitamin carrier. In: Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 915 (2). pp. 141-148.

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Official URL: http://dx.doi.org/10.1016/0167-4838(87)90293-7

Abstract

A specific protein exhibiting immunological cross-reactivity with chicken egg-white riboflavin carrier protein was detected by radioimmunoassay in the pregnancy sera of bonnet monkeys (Macaca radiata). Tiffs protein, which is capable of binding [14C]riboflavin, was purified by gel filtration on Sephacryl 8-300, fast protein liquid chromatography on a Mono Q anion exchanger and chromatofocusing on Mono P columns. The isolated primate carrier protein was similar to its avian counterpart in terms of physicochemicai characteristics, such as isoelectric point (pl < 4), electrophoretic mobility, molecular weight (approx. 36 000) and iigand binding. These findings may account for the extensive immunological cross-reactivity observed between the two proteins and suggest that the two vitamin carriers may have similar function in terms of embryonic vitamin nutrition.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Riboflavin carrier protein;Serum;Chromatography;fast protein liquid;Immunochemistry;(Chicken egg-white);Pregnant monkey
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 14 Mar 2008
Last Modified: 01 Mar 2012 07:27
URI: http://eprints.iisc.ernet.in/id/eprint/13257

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