Sadhu, Chanchal and Dutta, Santanu and Gopinathan, KP (1984) Influence of formamide on the thermal stability of DNA. In: Journal of Biosciences, 6 (6). pp. 817-821.
The utility of formamide in the denaturation and renaturation of DNA has been examined. The mmelting temperature of duplex DNA is lowered by 0.6°C per per cent of formamide. The depression of melting temperature is independent of the GC content. Formamide also increases the width of the thermal transition. Upto 30% it does not affect the rate of DNA reassociation.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Indian Academy of Sciences.|
|Keywords:||DNA melting;formamide;Tm;DNA base composition;DNA reassociation|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Microbiology & Cell Biology
|Date Deposited:||20 Mar 2008|
|Last Modified:||19 Sep 2010 04:43|
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