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The Rna Binding Subset of Adenosine Antibodies

Raju, Ramaswamy and Jacob, Mathai T (1986) The Rna Binding Subset of Adenosine Antibodies. In: Immunological Investigations, 15 (5). 405 -417.

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Abstract

Hapten specific antibodies were elicited against adenosine- BSA conjugate $(Ado^*-BSA)$ in rats. By radiohapten assay, two major populations of hapten specific antibodies were identified. They were directed against either adenosine trialcohol$(A^{ox-red})$ or morpholino-adenosine(Morph- de). The association constant (Ka) for the $^3H-A^{ox-red}$ binding population was determined to be 1.02 × $10^7 M^{-1}$. Majority of the $A^{ox-red}$ specific antibodies were crossreactive with Morph-Ade, but they did not cross-react at all with adenosine(A) or deoxyadenosine(dA). $^3H-A$ binding subsets were actually Morph-Ade specific and constitute less than 10% of the hapten specific antibodies. The Ka value for the $^3H-A$ binding was determined to be 4.5 × $10^6 M^{-1}$. A very minor and highly cross-reactive subset of Morph-Ade specific antibodies participate in RNA binding. Though a larger proportion of Morph-Ade specific antibodies interact with $^3H-A$, only a fraction of them bind to RNA. The extreme crossreactivity and hence, the highly adaptive nature of the binding sites of the antibodies interacting with RNA, might be a stringent requirement for recognising adenine residues of nucleic acids in solution.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Taylor & Francis.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 01 Apr 2008
Last Modified: 27 Aug 2008 13:18
URI: http://eprints.iisc.ernet.in/id/eprint/13552

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