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Separation of protocatechuate and homoprotocatechuate by paper chromatography and estimation of protocatechuate by colorimetry using a new $FeCl_3$ reagent

Raju, Ganapathi S and Khan, Nayeem U and Vaidyanathan, CS (1983) Separation of protocatechuate and homoprotocatechuate by paper chromatography and estimation of protocatechuate by colorimetry using a new $FeCl_3$ reagent. In: Journal of the Indian Institute of Science, 64 (3). pp. 1-10.

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Abstract

Protocatechuic acid (I), homoprotocatechuic acid (II), and protocatechuic aldehyde (III) were sepd. by paper chromatog. on Whatman no. 1 filter paper and then detected with a new reagent contg. $FeCl_3$ and $NH_4$ molybdate. Six solvent systems were tested, and the best results were obtained with 2% $HCOOH$. On the chromatograms, I and III gave bluish green spots, and II gave a dark brown spot. This new $FeCl_3-NH_4$ molybdate reagent was also used successfully for detn. of I by photometry. The absorbance was linearly related to 10-100 \mug

Item Type: Journal Article
Additional Information: Copyright of this article belongs to The Indian Institute of Science (IISc)
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 04 Apr 2008
Last Modified: 27 Aug 2008 13:18
URI: http://eprints.iisc.ernet.in/id/eprint/13561

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