Bhaduri, Tisha and Nagaraja, V (1994) DNA topoisomerase I from Mycobacterium smegmatis. In: Indian Journal of Biochemistry & Biophysics, 31 . pp. 339-343.
DNA topoisomerase I has been purified from Mycobacterium smegmatis to near homogeneity using different column chromatographic techniques. The enzyme activity relaxes form I DNA into form IV DNA, requiring Mg +2, but not ATP or any other cofactors for its activity. Several properties of the enzyme were found to be similar to that of the prototype enzyme, Escherichia coli topoisomerase I.
|Item Type:||Journal Article|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||01 Sep 2004|
|Last Modified:||19 Sep 2010 04:14|
Actions (login required)