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Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA

Achwal, Chhaya W and Iyer, Chitra A and Chandra, Sharat H (1983) Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA. In: FEBS Letters, 158 (2). pp. 353-358.

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Official URL: http://dx.doi.org/10.1016/0014-5793(83)80612-7

Abstract

We have reported that production and characterization of antibodies highly specific to 5-methyl-cytosine (5mC) and the development of a sensitive immunochemical method for the detection of 5mC in DNA [FEBS Lett. (1982) 150, 469]. Extension of this method to two other modified bases, 6-methyladenine (6mA) and 7-methylguanine (7mG), is reported here. By use of this immunochemical approach, we are able to detect 5mC, 6mA and 7mG in human and Drosophila DNA and confirm their presence in the DNA of two mealybug species

Item Type: Journal Article
Additional Information: Copyright of this aritcle belongs to Elsevier Science
Keywords: DNA methylation;Biotin—avidin cross-linking;DNA—protein interaction;Gene regulation Z-DNA;X-chromosome inactivation
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 03 Apr 2008
Last Modified: 24 Feb 2012 08:52
URI: http://eprints.iisc.ernet.in/id/eprint/13619

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