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Hemin-mediated oxidation of dithiothreitol reduces oxygen to $H_20$

Devi, Usha S and Ramasarma, T (1987) Hemin-mediated oxidation of dithiothreitol reduces oxygen to $H_20$. In: Molecular and Cellular Biochemistry, 77 (2). pp. 111-120.

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Abstract

Hemin catalyses the oxidation of dithiothreitol. One mole of oxygen is consumed for every 2 moles of dithiothreitol oxidized and the product is shown by spectral studies to be the intramolecular disulphide. The reaction shows a specificity for dithiol and for free heme moieties. Hemin molecules exhibit cooperativity in oxygen reduction. Oxygen radicals do not seem to be involved. $H_20_2$ is not required for this oxidation of dithiothreitol and does not appear to be an intermediate in the reduction of $O_2$ to $H_20$. However, an independent minor reaction involving a 2-electron transfer with the formation of $H_20_2$ also occurs. These studies on the hemin-catalyzed oxidation of dithiothreitol provide a chemical model for a direct 4-electron reduction of $O_2$ to $H_20$.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Springer.
Keywords: hemin catalysis;DTT oxidation;oxygen reduction.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 02 Apr 2008
Last Modified: 19 Sep 2010 04:44
URI: http://eprints.iisc.ernet.in/id/eprint/13633

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