Unni, Emmanual and Rao, MRS and Ganguly, J (1983) Histological and ultrastructural studies on the effect of vitamin A depletion and subsequent repletion with vitamin A on germ cells and Sertoli cells in rat testis. In: Indian Journal of Experimental Biology, 21 (4). pp. 180-192.Full text not available from this repository. (Request a copy)
At the onset of vitamin A [68-26-8] deficiency the testis Sertoli cell-spermatid assocn. was disrupted, resulting in sloughing off of the germ cells from the germinal epithelium. At the acutely deficient stage the seminiferous tubules were left with a single layer of spermatogonia and a no. of giant cells together with disorganized Sertoli cells. Even though B-type spermatogonia and preleptolene spermatocytes were present in the testis of rats maintained on retinoic acid, many of these were in a degenerated state. Peroxidase perfusion studies showed that the tracer penetrated into the lumen of the seminiferous tubules of the mild deficient and that of the retinoic-acid maintained rats while it was mostly concd. at the periphery of the tubules of the normal rat testes, thereby indicating disruption of the Sertoli-Sertoli cell junctional complexes and consequently the blood-testis barrier. Supplementation of the retinoate-fed rats with retinyl acetate led to several changes in the seminiferous tubules within 4 days such as (1) thinning of the germinal epithelium immediately after the supplementation; (2) re-establishment of the Sertoli-Sertoli cell junctional complexes; (3) cannanulation of the lumen of the tubules; and (4) appearance of pachytene spermatocytes. Examn. of the mitotic activity of the spermatogonial compartment revealed marked redn. in the activity in acutely deficient animals while the retinoate-fed rats it was slightly lower than in controls. Supplementation of the retinoate-treated rats with retinyl acetat led to a gradual increase, with the progress of time in the percentage of the spermatogonia in mitosis.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to National Institute of Science Communication and Information Resources|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||16 Apr 2008|
|Last Modified:||27 Aug 2008 13:19|
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