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Cloning of rice DNA and identification of the cloned $H_2A$, $H_2B$, and $H_4$ histone genes

Thomas, G and Vasavada, HA and Sachariah, E and Padayatty, JD (1983) Cloning of rice DNA and identification of the cloned $H_2A$, $H_2B$, and $H_4$ histone genes. In: Indian Journal of Biochemistry & Biophysics, 20 (1). pp. 8-15.

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Abstract

DNA isolated from 48-h germinated rice embryos and cut with restriction endonuclease BamHI was inserted at the BamHI site in plasmid pBR322 and cloned in Escherichia coli HB101. Recombinantion of DNA-contg. clones were selected by their resistance to ampicillin and sensitivity to tetracycline and identified by colony hybridization to homologous probes comprising 32P-labeled RNA from 18-h germinated rice embryos. Two clones contg. genes for 9-12 S poly(A)- RNA, which was enriched in histone messengers, were identified. One of the clones contained an insert of 6.64 kilobases. RNAs that hybridized with this insert were translated into $H_2A4, 4H_2B$, and $H_4$ histones. Thus, the inserted DNA contains these genes.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to National Institute of Science Communication and Information Resources
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 15 Apr 2008
Last Modified: 27 Aug 2008 13:19
URI: http://eprints.iisc.ernet.in/id/eprint/13675

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