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Existence of single-strand interruptions in the genomic DNA of mycobacteriophage I3

Reddy, Bramham A and Gopinathan, KP (1986) Existence of single-strand interruptions in the genomic DNA of mycobacteriophage I3. In: FEMS Microbiology Letters, 37 (2). pp. 163-167.

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Abstract

The genomic DNA of mycobacteriophage I3 harbours single-strand interruptions and is susceptible to degradation by nuclease S1, leading to random fragmentation. The native DNA serves as an efficient template for DNA polymerase I showing the presence of free 3'-OH termini which provide the priming points within the genome. The single-strand regions show no specificity for the 5'-end nucleotides flanking these interruptions and all 4 nucleotides are present with a probability equivalent to their percentage in the total DNA. The single-strand interruptions are an intrinsic character of this genome and are present irrespective of the host on which the phage is propagated.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Blackwell Synergy.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 25 Apr 2008
Last Modified: 27 Aug 2008 13:20
URI: http://eprints.iisc.ernet.in/id/eprint/13767

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