Rao, Sankara K (1986) Plantlets from somatic callus tissue of the East Indian Rosewood (Dalbergia latif olia Roxb.). In: Plant Cell Reports, 5 (3). pp. 199-201.
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Callus-mediated shoot bud formation was demonstrated in Dalbergia latifolia Roxb. (East Indian Rosewood). Cultures were raised from shoot explants of six year-old plants on Murashige and Skoog (MS) medium supplemented with naphthaleneacetic acid (NAA) and benzyladenine (BA). A sequential treatment of callus with increasing BA levels and decreasing NAA ensured shoot bud induction. Rooting of shoots was achieved by a three-step culture procedure involving 1) White's(W) liquid medium containing indoleacetic acid (IAA), naphthaleneacetic acid and indolebutyric acid (IBA), 2) half-strength MS agar-solidified medium with charcoal (0.25%) and 3) half-strength MS liquid medium.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Springer.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||24 Apr 2008|
|Last Modified:||19 Sep 2010 04:44|
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