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Inhibition of mitochondrial electron transport and energy transduction by 2-methyl-4-dimethylaminoazobenzene in vitro

Saikumar, P and Kurup, Ramakrishna CK (1984) Inhibition of mitochondrial electron transport and energy transduction by 2-methyl-4-dimethylaminoazobenzene in vitro. In: Indian Journal of Biochemistry & Biophysics, 21 (5). pp. 309-313.

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Abstract

2-Methyl-4-dimethylaminoazobenzene (I) [54-88-6] inhibited electron transport and phosphorylation in tightly coupled rat liver mitochondria. It stimulated state 4 respiration, decreased ADP/O ratio, inhibited state 3 oxidation and abolished respiratory control. These effects reveal its action as an inhibitor uncoupler. Consistent with its preferential inhibition of the NADH-ubiquinone segment of the respiratory chain, ferricyanide reduction was far more sensitive to inhibition by I when NAD+-linked substrates were used as electron donors than when succinate was used. Like a true uncoupler, it stimulated the dormant ATPase [9000-83-3] activity in tightly coupled mitochondria in the presence and absence of Mg. However, in the absence of added Mg, dinitrophenol-stimulated activity was drastically inhibited indicating its action as a membrane destabilizing agent. In sonic submitochondrial particles, I inhibited energy-linked reverse electron transport and transhydrogenase reaction.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to The Council of Scientific & Industrial Research.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 07 May 2008
Last Modified: 27 Aug 2008 13:21
URI: http://eprints.iisc.ernet.in/id/eprint/13842

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