ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Characterization of Mycobacteriophage I8 and its Unrelatedness to Mycobacteriophages I1, I3 and I5

Reddy, Bramham A and Gopinathan, KP (1987) Characterization of Mycobacteriophage I8 and its Unrelatedness to Mycobacteriophages I1, I3 and I5. In: Journal of General Virology, 68 (4). pp. 949-956.

[img] PDF
1987_JGV_V68_pp949-956.pdf
Restricted to Registered users only

Download (3196Kb) | Request a copy

Abstract

Homology among the genomes of mycobacteriophages I1, I3, I5 and I8 has been studied. Based on restriction endonuclease cleavage patterns, dot blot hybridization and Southern blot hybridization analysis, the DNAs of phages I1, I3 and I5 have been shown to be homologous and indistinguishable, but entirely different from phage I8. Unlike the others, the I8 genome does not harbour any single-strand interruptions. The DNA is 43 kb in length with limited cyclic permutations and has a G + C content of 54%. The presence of 5-methylcytosine in I8 DNA was indicated from the restriction patterns of MspI and HpaII. The number of sites and fragment sizes for several restriction enzymes on I8 DNA has been determined. Phage I8 has a replication cycle of 300 min, with a latent period of 180 min, a rise period of 120 min and a burst size of 100. The viability of phage I8 is significantly reduced by treatment with organic solvents.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Society for General Microbiology.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 05 May 2008
Last Modified: 19 Sep 2010 04:44
URI: http://eprints.iisc.ernet.in/id/eprint/13845

Actions (login required)

View Item View Item