Recognition of B and Z forms of DNA by Escherichia coli DNA polymerase I

Ramesh, N and Shouche, Yogesh S and Brahmachari, Samir K (1986) Recognition of B and Z forms of DNA by Escherichia coli DNA polymerase I. In: Journal of Molecular Biology, 190 (4). pp. 635-638.

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Abstract

Since the substrate binding domain of the large proteolytic fragment of E.coli DNA polymerase I has been shown to interact with the B forms of DNA, the ability of this enzyme to recognize structures other than the B form was studied.The polymerase activity has been used to evaluate the degree of recognition of the B and Z forms of DNA.The Z form was found to promote less activity, indicating the probable inability of the polymerase to move along the conformationally rigid form of the template.The present study indicates that the Z-DNA found in vivo may have a role in the control of replication.

Item Type:	Journal Article
Additional Information:	Copyright belongs to Elsevier Ltd.
Keywords:	DNA polymerase
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	09 May 2008
Last Modified:	19 Sep 2010 04:44
URI:	http://eprints.iisc.ernet.in/id/eprint/13913

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