Desai, Kartiki V and Flanders, Kathleen C and Kondaiah, Paturu (1998) Expression of transforming growth factor-beta isoforms in the rat male accessory sex organs and epididymis. In: Cell and Tissue Research, 294 (2). pp. 271-277.
We studied the expression and distribution of transforming growth factor-beta (TGF-beta) isoforms in the rat male accessory sex glands and the epididymis. Our data demonstrate the expression of both TGF-beta 1 and -beta 3 isoforms in ventral prostate (VP), seminal vesicle (SV), coagulating gland (CG), and epididymis (E) by Northern blot analysis. In addition, there was differential expression of TGF-beta 3 in the three regions of epididymis, the corpus region being the highest. Immunostaining data showed intense staining for latent TGF-beta 1 in all the male accessory glands. In contrast, no staining using antibodies specific for active TGF-beta 1 was observed. No expression of TGF-beta 2 was evident either by immunohistochemistry or Northern blot analysis. The presence of mature TGF-beta 3 protein was observed in the secretory epithelium of VP, CG, and corpus E. There was no detectable staining of TGF-beta 3 in the seminal vesicle and caput and cauda regions of epididymis. These data suggest possible differential regulation of TGF-beta isoform expression in the male reproductive system and predict unique roles for individual TGF-beta isoforms in sperm maturation and maintenance.
|Item Type:||Journal Article|
|Additional Information:||Copyright for this article belongs to Springer-Verlag.|
|Keywords:||Coagulating gland;Epididymis;Gene expression;Prostate;Seminal vesicle;Rat (Wistar)|
|Department/Centre:||Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)|
|Date Deposited:||14 Sep 2004|
|Last Modified:||19 Sep 2010 04:15|
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