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# Maintenance of antigen-specific immunological memory through variable regions of heavy and light chains of anti-idiotypic antibody

Vani, J and Nayak, R and Shaila, MS (2007) Maintenance of antigen-specific immunological memory through variable regions of heavy and light chains of anti-idiotypic antibody. In: Immunology, 120 (4). pp. 486-496.

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## Abstract

Immunological memory is characterized by a quick and enhanced immune response after re-exposure to the same antigen. To explain the mechanism involved in generation and maintenance of immunological memory, we had earlier proposed a hypothesis involving the relay of memory by idiotypic and anti-idiotypic B cells. The peptidomimic present in the hypervariable region of anti-idiotypic antibody was hypothesized to carry forward immunological memory. In the present work, we provide evidence supporting a role for the anti-idiotypic antibody in eliciting antigen-specific B-cell and T-cell responses. Employing the idiotypic monoclonal antibody $(Ab_1)$ specific for haemagglutinin (H) protein of rinderpest virus, $Ab_{2\beta}$ was generated, which possesses an internal image of the H protein in the region between amino acids 527 and 556. We demonstrate that antigen-specific memory is perpetuated by immunization with $Ab_2$, as shown by maintenance of antigen-specific T-cell responses upon restimulation in vitro of $Ab_2$ immune splenocytes by antigen-presenting cells expressing H protein or pulsed with H-protein-derived peptides. We have also shown that boosting with antigen-specific anti-idiotypic B cells generates a memory response in antigen-primed mice. Evidence has been provided for the existence of an antigen-specific B-cell idiotypic network in the body that supports the perpetuation of immunological memory as proposed in the relay hypothesis.

Item Type: Journal Article Copyright of this article belongs to Blackwell Publishing Ltd. anti-idiotypic-B cells;idiopeptide;immunological memory;peptidomimics; relay hypothesis Division of Biological Sciences > Microbiology & Cell Biology 29 May 2008 19 Sep 2010 04:45 http://eprints.iisc.ernet.in/id/eprint/14089

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