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Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum

Swarnamukhi, Pidugu L and Sharma, Shailendra K and Padala, Prasanth and Surolia, Namita and Surolia, Avadhesha and Suguna, Kaza (2007) Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum. In: Acta Crystallographica Section D Biological Crystallography, 63 (4). pp. 458-464.

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Abstract

The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited nonisomorphism. The c axis of the I222 cell showed a large variation of about $16 \AA$ , from $c = 81 \AA$ to $c = 97 \AA$. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, $c = 97 \AA$; form 2, $c = 92 \AA$; form 3, $c = 81 \AA$). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to International Union of Crystallography.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 03 Jun 2008
Last Modified: 19 Sep 2010 04:45
URI: http://eprints.iisc.ernet.in/id/eprint/14146

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