Swarnamukhi, Pidugu L and Sharma, Shailendra K and Padala, Prasanth and Surolia, Namita and Surolia, Avadhesha and Suguna, Kaza (2007) Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum. In: Acta Crystallographica Section D Biological Crystallography, 63 (4). pp. 458-464.
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The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited nonisomorphism. The c axis of the I222 cell showed a large variation of about $16 \AA$ , from $c = 81 \AA$ to $c = 97 \AA$. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, $c = 97 \AA$; form 2, $c = 92 \AA$; form 3, $c = 81 \AA$). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to International Union of Crystallography.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||03 Jun 2008|
|Last Modified:||19 Sep 2010 04:45|
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