Yadav, V and Sudhagar, R and Medhamurthy, R (2002) Apoptosis During Spontaneous and Prostaglandin F2a-Induced Luteal Regression in the Buffalo Cow (Bubalus bubalis): Involvement of Mitogen-Activated Protein Kinases. In: Biology of Reproduction, 67 (3). pp. 752-759.
The present study was conducted to evaluate whether corpus luteum (CL) of the buffalo cow undergoes luteal regression by the process of apoptosis, and to examine the involvement of mitogen-activated protein (MAP) kinases during PGF2a-induced luteolysis. Sections of CL from late-estrous cycle i.e., during spontaneous luteolysis, stained for DAPI revealed increased number of condensed nuclei indicating cell death by apoptosis confirmed further by the occurrence of pronounced oligonucleosome formation. For morphological and biochemical characterization during PGF2a-induced apoptosis, CL were collected at 0, 4, 12 and 18 h after injection of 750 mg of Tiaprost, a synthetic analog of PGF2a to mid-estrous cycle buffalo cows. Serum P4 concentrations fell within 4 h and decreased (p < 0.05) maximally by 18 h. A concomitant decrease (p < 0.05) in the levels of StAR mRNA and protein were observed in CL during 12-18 h, with profound effect on mRNA levels. Quantitative analysis of the genomic DNA showed a >5 fold increase (p < 0.05) in the low molecular weight DNA fragments by 18 h post injection. Immunoblot analysis of CL tissue lysates showed increased (p < 0.05) levels of phospho-JNK1 (4-14 fold during 4-18 h) and phospho-p38 (2-4 fold at 18 h). Immunohistochemistry of CL sections revealed an increased nuclear localization of phospho-JNK after treatment. These findings demonstrate that CL of the buffalo cow undergoes cell death by the process of apoptosis both during spontaneous and PGF2a-induced luteolysis, and that MAP kinases are involved during PGF2a-mediated apoptosis in the CL.
|Item Type:||Journal Article|
|Additional Information:||First demonstration of activation of JNK arm of MAP kinases during corpus Luteum Cell death in vivo|
|Keywords:||Apoptosis;Corpus Luteum;ovary;MAP kinases;StAR|
|Department/Centre:||Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)|
|Date Deposited:||16 Feb 2005|
|Last Modified:||16 Jan 2013 11:32|
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