Periannan, Senapathy and Mir, Ahmed A and Jacob, Mathai T (1985) Mechanism of coupling periodate-oxidized nucleosides to proteins. In: FEBS Letters, 190 (2). pp. 337-341.
This paper describes a mechanism of coupling periodate-oxidized nucleosides to proteins. Each of the dialdehyde groups of a periodate-oxidized nucleoside is shown to couple to lysine residues on different protein molecules through Schiff bases, thereby cross-linking different protein molecules, forming a polymer. This is in contrast to the previous model in which nuclqosides were suggested to couple to proteins through a morpholine structure. The cross-linked structure of the nucleoside-antigen, significantly different when compared to the native protein, may affect the specificity and the efficiency of antibody production.
|Item Type:||Journal Article|
|Additional Information:||Copyright belongs to Elsevier Science|
|Keywords:||Nucleoside-antigen Periodate oxidation Schiff base Protein cross-linking Polymerization;Antibody production|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||11 Jun 2008|
|Last Modified:||19 Sep 2010 04:45|
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