Hariharan, N and Padayatty, JD (1987) Ribosomal RNA gene repeat units in rice embryos. In: Indian Journal of Biochemistry & Biophysics, 24 (3). pp. 157-164.Full text not available from this repository. (Request a copy)
The hybridization of 32P-labeled 25 S, 17 S and 5.8 S rRNAs with the discrete EcoRI, BglII, or BamHI 8.8-kb band from the rice variety IR20 DNA showed the presence of EcoRI, BglII, or BamHI families of tandemly repeated rDNA units. The BamHI digestion of rice DNA also produced 3.8- and 5.0-kb bands which are derived from the major 8.8-kb BamHI fragment. The 3.8-kb DNA cloned in pBR322 at the BamHI site, hybridized pos. with 25 S, 17 S and 5.8 S rRNAs sep., showing the presence of complementary sequences for these rRNAs. The partial nucleotide sequences of the 3.8-kb restriction fragments were identical with the rRNA gene sequences of rice variety Mangetsumochi, with some deletions, addns., and substitutions in the 17 S and 25 S rRNA genes in rice variety IR20. Nucleotide sequence anal. showed that the rRNA genes are organized in the order 17 S-5.8 S-25 S.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to National Institute of Science Communication and Information Resources.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||17 Jun 2008|
|Last Modified:||27 Aug 2008 13:29|
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