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Fractionation and identification of cytoplasmic tRNAs and structural characterization of a phenylalanine and a leucine tRNA from cucumber hypocotyls

Jayabaskaran, C and Puttaraju, M (1993) Fractionation and identification of cytoplasmic tRNAs and structural characterization of a phenylalanine and a leucine tRNA from cucumber hypocotyls. In: Biochemistry and Molecular Biology International, 31 (5). pp. 983-995.

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Abstract

Total cytoplasmic tRNAs from cucumber hypocotyls were fractionated by two-dimensional polyacrylamide gel electrophoresis into about 56 species. Of these, 32 tRNA species were identified by aminoacylation as tRNA specific for 15 amino acids. Furthermore, cytoplasmic tRNAPhe and tRNALeu (NAA) were purified to homogeneity by the combination of RPC-5 column chromatography and two-dimensional polyacrylamide gel electrophoresis. The primary structure of tRNALeu(NAA) and the modified nucleotides of tRNAPhe have been determined. The sequence of cucumber cytoplasmic tRNALeu(NAA) is identical to that of bean cytoplasmic tRNALeu(NAA) except for three base differences in the variable loop.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Taylor and Francis. The Journal Biochemistry and Molecular Biology International is now known as HealthCare Life.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 01 Jul 2008
Last Modified: 27 Aug 2008 13:30
URI: http://eprints.iisc.ernet.in/id/eprint/14614

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