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Alterations in thermal stability of glutamine synthetase and aspartate transcarbamylase in finger millet (Eleusine coracana) seedlings subjected to water deprivation

Bharathalakshmi, * and Savithri, HS and Rao, Appaji N and Vidyanathan, CS (1987) Alterations in thermal stability of glutamine synthetase and aspartate transcarbamylase in finger millet (Eleusine coracana) seedlings subjected to water deprivation. In: Biochemistry International, 15 (1). pp. 235-243.

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Abstract

Thermal inactivation and urea denaturation profiles of partially purified glutamine synthetase from normal and water-stressed finger millet (E. coracana) seedlings were compared. The enzyme from stressed seedlings was more stable to heat inactivation and urea denaturation,suggesting that the enzyme structure was probably altered in response to water deprivation. Thermal stability studies of aspartate transcarbamylase from normal and water-stressed finger millet seedlings showed enhanced activities of the enzyme upon heating. Although this enhancement was lower in the stressed than in the normal enzyme, the presence of L-proline brought the activity of the enzyme from stressed seedlings on par with that of the normal enzymes.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to International Union of Biochemistry- Academic Press.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 11 Aug 2008
Last Modified: 27 Aug 2008 13:33
URI: http://eprints.iisc.ernet.in/id/eprint/14908

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