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A novel association of a polymorphism in the first intron of adiponectin gene with type 2 diabetes, obesity and hypoadiponectinemia in Asian Indians

Vimaleswaran, Karani S and Radha, Venkatesan and Ramya, Kandaswamy and Babu, Hunsur Narayan Sathish and Savitha, Nageshappa and Roopa, Venkataramaiah and Monalisa, Dhar and Deepa, Raj and Ghosh, Saurabh and Majumder, Partha P and Rao, MR Sathyanarayana and Mohan, Viswanathan (2008) A novel association of a polymorphism in the first intron of adiponectin gene with type 2 diabetes, obesity and hypoadiponectinemia in Asian Indians. In: Human Genetics, 123 (6). pp. 599-605.

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Abstract

Adiponectin is an adipose tissue specifc protein that is decreased in subjects with obesity and type 2 diabetes. The objective of the present study was to examine whether variants in the regulatory regions of the adiponectin gene contribute to type 2 diabetes in Asian Indians.The study comprised of 2,000 normal glucose tolerant (NGT)and 2,000 type 2 diabetic, unrelated subjects randomly selected from the Chennai Urban Rural Epidemiology Study (CURES), in southern India. Fasting serum adiponectin levels were measured by radioimmunoassay. We identiWed two proximal promoter SNPs (-11377C \rightarrow G and -11282T \rightarrow C), one intronic SNP (+10211T \rightarrow G)and one exonic SNP (+45T!G) by SSCP and direct sequencing in a pilot study (n = 500). The +10211T \rightarrow G SNP alone was genotyped using PCR-RFLP in 4,000 study subjects. Logistic regression analysis revealed that subjects with TG genotype of +10211T \rightarrow G had signiWcantly higher risk for diabetes compared to TT genotype [Odds ratio 1.28; 95% ConWdence Interval (CI) 1.07–1.54; P = 0.008]. However, no association with diabetes was observed with GG genotype P = 0.22). Stratification of the study subjects based on BMI showed that the odds ratio for obesity for the TG genotype was 1.53 (95%CI 1.3–1.8; $P < 10^{-7}$) and that for GG genotype, 2.10 (95% CI 1.3–3.3; P = 0.002). Among NGT subjects, the mean serum adiponectin levels were signiWcantly lower among the GG (P = 0.007) and TG(P = 0.001) genotypes compared to TT genotype. Among Asian Indians there is an association of +10211T \rightarrow G polymorphism in the Wrst intron of the adiponectin gene with type 2 diabetes, obesity and hypoadiponectinemia.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Springer.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 21 Jul 2008
Last Modified: 19 Sep 2010 04:47
URI: http://eprints.iisc.ernet.in/id/eprint/14945

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