Rao, Narasinga BN and Kumar, Anil and Balaram, Hemalatha and Ravi, A and Balaram, P (1983) Nuclear Overhauser Effects and circular Dichroism as Probes of \beta.-turn Conformations in Acyclic and Cyclic Peptides with Pro-X Sequences. In: Journal of the American Chemical Society, 105 (25). pp. 7423-7428.
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Nuclear Overhauser effects (NOE) and (CD) techniques have been used to probe \beta-turn conformations in acyclic and cyclic peptides containing Pro-X sequences. The model peptides studied are of the type Piv-Pro-X-NHMe (X = Aib, D-Ala, Gly, Val, and Leu) and Boc-Cys-Pro-X-Cys NHMe (X = Aib, L-Ala, D-Ala, Gly, and Leu). In the acyclic series, observation of NOE's between Pro $C^\alphaH$ and X-NH, together with solvent and temperature dependence of NH chemical shifts, establishes a 4 \longrightarrow 1 hydrogen bond stabilized type I1 \beta-turn in the Gly, D-Ala, and Aib peptides, in $CDC1_3$ and $(CD_3)_2S0$. A positive $n-\pi^*$ CD band at \sim 225-230 nm appears to be characteristic of this structure. For the acyclic Pro-Leu peptide the observation of NOE's for both Pro and Leu $C^\alphaH$ resonances on saturation of Leu NH is compatible with a type V bend or consecutive \gamma-turn conformation. In the cyclic disulfide series the Pro-Aib and Pro-D-Ala peptides favor type I1 \beta-turns, whereas all other peptides adopt type I (111) conformations. All the cyclic disulfides exhibit an intense negative CD band at \sim 228-230 nm. The results suggest that general correlations between CD spectral type and specific \beta-turn conformations may not be obtained. Evidence for solvent-dependent structural changes in the Pro-Aib sequence in both cyclic and acyclic peptides is presented.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to American Chemical Society.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit
Division of Physical & Mathematical Sciences > Physics
|Date Deposited:||14 Jul 2008|
|Last Modified:||19 Sep 2010 04:47|
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