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Correlation between riboflavin carrier protein induction and its $mRNA$ activity in estrogen stimulated chicken liver and oviduct

Kumari, Durga B and Adiga, PR (1986) Correlation between riboflavin carrier protein induction and its $mRNA$ activity in estrogen stimulated chicken liver and oviduct. In: Journal of Biosciences, 10 (2). pp. 193-202.

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Abstract

Poly(A)-enriched $RNA$ from either liver or oviduct of estradiol -17/beta treated immature chicks supported $[3H]$ leucine incorporation into immunoprecipitable riboflavin-carrier protein in a dose-dependent manner when translated in the rabbit reticulocyte lysate system. Primary translation product of riboflavin-carrier protein had a molecular weight of 38,000, which on incubation with a stripped hepatic microsomal preparation was processed to a product with a size comparable to native riboflavin-carrier protein. Poly(A)-enriched $RNA$ from both the liver and the oviduct of estrogen-treated birds stimulated $[^3H]$ -leucine incorporation into riboflavin-carrier protein, and this was 2-3-fold higher during secondary stimulation vis-a-vis primary stimulation with the steroid. Poly A-enriched RNA from the liver of progesterone -treated birds during secondary stimulation did not support riboflavin-carrier protein synthesis. In contrast, poly A-enriched $RNA$ from the oviduct of the birds treated with progesterone during secondary (but not primary) stimulation did exhibit riboflavin-carrier protein-$mRNA$ activity which was comparable to that stimulated by estradiol- 17\beta.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Indian Academy of Sciences.
Keywords: Primary translation product;cell-free translation;rabbit reticulocyte lysate;stripped microsomal membrane; progesterone;estradiol- 17β;precursor;poly A + -RNA.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 17 Jul 2008
Last Modified: 01 Mar 2012 06:06
URI: http://eprints.iisc.ernet.in/id/eprint/15042

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