Chandrashekaran, Siddamadappa and Shankar, Aparna Bhavani and Babu, Padmanabhan and Paul, Bindu Diana and Nagaraja, Valakunja (1999) Identification and characterization of a type II restriction endonuclease, Stri from streptomyces thermodiastaticus. In: Current Science, 77 (2). pp. 273-276.
A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5-C down arrow TCGAG-3 as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.
|Item Type:||Journal Article|
|Additional Information:||The copyright belongs to Indian Academy of Sciences.|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||07 Mar 2005|
|Last Modified:||19 Sep 2010 04:15|
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