Devireddy, LR and Raghavan, R and Ramachandran, S and Shaila, MS (1999) The fusion protein of peste des petits ruminants virus is a hemolysin. In: Archives Of Virology, 144 (6). pp. 1241-1247.
The fusion glycoprotein (F protein) of paramyxoviruses plays a vital role in virus-induced cytopathology. To explore the role of the F protein in peste des petits ruminants virus (PPRV)- induced cytopathology, the F protein of PPRV was purified by immunoaffinity chromatography. The purified F protein, when incubated with chicken erythrocytes, caused lysis suggesting that PPRV F protein is a hemolysin. Furthermore, the hemolysis can be inhibited by hyperimmune serum against F protein. The virus-induced cell fusion (syncytia) was also inhibited by the hyperimmune serum against the F protein. In summary, these results indicate that the purified PPRV F protein is biologically active and is involved in virus-induced hemolysis, cell-fusion and the initiation of infection.
|Item Type:||Journal Article|
|Additional Information:||The copyright belongs to Springer Verlag.|
|Keywords:||fusion protein;petits ruminants virus;hemolysin;immunoaffinity chromatography|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||18 Mar 2005|
|Last Modified:||19 Sep 2010 04:15|
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